Frequency of Paroxysmal Nocturnal Hemoglobinuria in Patients with Lymphoma

Objective: To investigate the frequency of paroxysmal nocturnal hemoglobinuria (PNH), an acquired clonal hematopoietic stem cell disease resulting in complement-mediated hemolysis, patients with lymphoma by flow cytometry.Methods: Fifty who were admitted to hematology clinic, newly diagnosed and not yet treated included this study conducted 2014. The presence PNH clones was checked FLAER cytometry method peripheral blood samples. is a non-hemolytic fluorescently labeled inactive toxin aerolysin that can detect up 0.5% cells instead bacterial aerolysin, which binds RBCs via GPI anchor initiates hemolysis for screening or clone detection. With technique, all lines be detected assay.Results: observed over 10% two patients, one male other female. However, no found clones. subtypes positive B-cell small-cell lymphocytic patient primary splenic female patient.Conclusion: PNH-like disorders accompanying hematological malignancies, especially lymphomas, are very common literature. There need elucidate relationship between malignancies help more advanced molecular techniques.